Proteinase K
10mg/ml in 10mM Tris HCl pH 7.5
| ProK | 100mg |
| 1M Tris pH7.5 | 100µl |
| Clean H2O | 9.9ml |
Mix the Tris and water then add to the pot of ProK, shake until resuspended, aliquot into 1ml (in an eppie) and store at -20°C
1M Tris-HCL pH 7.5 or 8 (1L)
| Tris HCl (mw 121.1) | 121g |
| Clean H2O | 800ml |
Adjust pH to 7.5 or 8 using hydrochloric acid and then top up to one litre.
0.5M EDTA pH 8.0 (500ml)
| EDTA (mw 372.24) | 93.06g |
| Clean H2O | 250ml |
Adjust pH to 8 with NaOH pellets (you will need about about 20 g) or NaOH solution. When pH gets close the EDTA will dissolve, top up to 500ml with water.
RNAse A Solution
| RNAse A | 100mg |
| 1M Tris pH7.5 | 100µl |
| Clean H2O | 9.9ml |
AFter Aliqouting 1ml per eppie heat @98
oC for 5 minutes to remove any DNAses.
10% w/v SDS (sodium dodecyl sulphate) 500ml
| SDS (mw 288.4) | 50g |
| Clean H2O | 500ml |
Do not autoclave as polymers form.
Elution Buffer (500ml)
10mM Tris-Cl, pH 8.5
| 1M Tris-HCl pH 8.5 | 5ml |
| Clean H2O | 495ml |