PCR Calculator

PCR master mix calculator

µl Volume per reaction


number of reactions (include one for negative control and one for luck!)


IngredientStockDesired1 rxnMaster Mix
BufferX X µl µl
MgCl2mM mM µl µl
dNTPmM mM µl µl
Fwd PrimerµM µM µl µl
Rev PrimerµM µM µl µl
TaqU/µl U/rxn µl µl
DNA µl µl µl
H2O µl µl


Click here if buffer contains 1.5mM final MgCl2

Click here if using 50mM MgCl2 Stock

Click here if using 2.5mM DNTP Stock



Click this button to calculate the pcr





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